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Fluorescent image of 5,(6)-carboxy-2',7'dichlorofluorescein (CDF) in B-CLEAR® RT. The proportion of CDF found in the bile canaliculi (biliary excretion index) is approximately 35% and in vitro biliary clearance is approximately 8.2 mL/min/kg body mass.

Probe substrates using B-CLEAR® demonstrate a high in vitro to in vivo biliary clearance correlation in both rat and human studies.

B-CLEAR^®, a patented* and Transporter Certified ^† sandwich-cultured hepatocyte platform, is the only in vitro method to predict in vivo hepatic uptake, biliary excretion, and biliary clearance. B-CLEAR^® is the only system with certified uptake AND efflux transport.

B-CLEAR^® allows you to:

Differentiate compounds for candidate selection by rank order according to potential for biliary excretion, intracellular concentration, and degree of hepatic accumulation

Predict in vivo human biliary clearance potential prior to running clinical trials

Evaluate interspecies differences in liver uptake, hepatic accumulation, intracellular concentration, and biliary excretion

Identify drug-drug interactions related to hepatic uptake/efflux and assess possible clinical implications

Improve hepatotoxicity predictions and yield in vivo-relevant intracellular concentrations of your compound through the expression and function of uptake AND efflux transporters

Qualyst's mission is to develop and deliver quality products and data to clients based on our industry-leading expertise in drug transport and hepatic disposition.

B-CLEAR® is a registered trademark of Qualyst, Inc.

*B-CLEAR® is covered by US Patent 6,780,580 and other worldwide patents issued & pending.

^†Transporter Certified means that the specified product has been functionally evaluated for multiple uptake and efflux transporters and that the values determined fall within a defined range of acceptable values.

The International Transporter Consortium recognizes the importance of the sandwich-cultured hepatocyte model in drug development (Nat Rev Drug Discov. 2010 Mar;9(3):215-36). Read our article on drug transport and the utility of B-CLEAR^® in drug discovery and development here.